![]() Solids will impair the running of the gel and it is likely your protein of interest will remain in the stacking gel. If your protein of interest is in the insoluble fraction (e.g., cell membrane-bound proteins) investigate pretreatment methods to liberate and solubilize it first. For a clean image, samples are centrifuged to remove any solids, in order to load only the soluble fraction. The specific separation method chosen will depend on the aim of the analysis. This is typically achieved by protein electrophoresis, such as sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) or native PAGE, which separates proteins based on their molecular weight or charge. b) in situ hybridization 15.Figure 1: Overview of a western blot protocol.īefore a western blot can be performed, the proteins in the sample must be separated. b) polyacrylamide gel is commonly used 11. a) used to identify a specific protein 10. ![]() c) Initially fragments are separated by electrophoresis in northern hybridization 9. The separation technique of charged molecules under the influence of electric current is called a) colony hybridization b) electrophoresis c) dot blot technique d) western blotting The technique used to locate specific genes in chromosomes is a) colony hybridization b) in situ hybridization c) dot blot technique d) western blotting 15. ELISA is based on a) antigen-antibody interaction b) antigen-protein intercation c) lectin- antibody interaction d) all of these 14. For protein detection, most commonly used probe is a) antibody b) lectin c) antigens d) interferonsġ3. For glycoproteins, most commonly used probe is a) antibody b) lectin c) antigens d) interferons 12. In Western blotting a) agarose gel is commonly used b) polyacrylamide gel is commonly used c) both a and b d) high resolution gels 11. ![]() Sothern hybridization is a) used to identify a specific protein b) used to identify a specific DNA c) used to identify a specific RNA d) used to identify both DNA and RNA 10. All are differences in procedure between Northern and Southern hybridization except a) DBM membrane is used in northern hybridization b) RNA:DNA hybrids are formed in northern hybridization c) Intially fragments are separated by electrophoresis in northern hybridization d) DNA denaturation is required before blotting in Sothern hybridization 9. In Northern hybridization probe hybridization forms a) DNA:DNA hybrid b) RNA:DNA hybrid c) both a and b d) none of these 8. Northern hybridization is a) used to identify a specific protein b) used to identify a specific DNA c) used to identify a specific RNA d) used to identify both DNA and RNA 7. The technique used to detect the presence of DNA or RNA in a non-fractionated DNA sample is a) colony hybridization b) in situ hybridization c) dot blot technique d) western blotting 6. Applications of Southern blotting includes a) DNA fingerprinting b) preparation of RFLP maps c) identification of transferred genes d) all of these 5. Which of the following statements are true regarding southern blotting a) developed by E.M.Southern b) DNA-DNA hybridization is the basis c) The transfer of DNA fragments from gel to nitrocellulose membrane is called blotting d) all of these 4. Sothern hybridization is a) used to identify a specific protein b) used to identify a specific DNA c) used to identify a specific RNA d) used to identify both DNA and RNA 3. The technique used to identify specific DNA sequence in bacterial colonies is a) colony hybridization b) in situ hybridization c) dot blot technique d) western blotting 2. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |